Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

Mfr.No.

EH2622

Alternative Names

AMPK ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of AMPK and the recovery rates were calculated by comparing the measured value to the expected amount of AMPK in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-104	94
EDTA plasma(n=5)	89-100	94
heparin plasma(n=5)	92-105	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-105%	91-97%	88-104%
EDTA plasma(n=5)	85-99%	83-101%	86-98%
heparin plasma(n=5)	80-100%	84-94%	80-94%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

AMPK

Notes

For Research Use Only

UniProt ID	Q13131
Antigen	AMPK

Applications	ELISA
Applications Notes	AMPK ELISA Kit allows for the in vitro quantitative determination of AMPK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: The Journal of Maternal-Fetal & Neonatal Medicine Title: Maternal serum AMP-activated protein kinase levels in mild and severe preeclampsia

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801864	EH2622	96T	$400		Add to Cart

Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

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