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Home / ELISA Kit

Human Aminoacylase-1 (ACY1) ELISA Kit

SKU : BHE13703122

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids.
ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

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Mfr.No.	KTE60906
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Aminoacylase-1 (ACY1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACY1.
Alternative Names	ACY1; ACY1D; ACYLASE;
Detection Method	Colorimetric
Kit Components	Human Aminoacylase-1 microplate Human Aminoacylase-1 standard Human Aminoacylase-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Aminoacylase-1 (ACY1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACY1. No significant cross-reactivity or interference between Human ACY1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
Notes	This Human Aminoacylase-1 (ACY1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACY1 in samples. An antibody specific for ACY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	95

Applications	ELISA
Applications Notes	This Human Aminoacylase-1 (ACY1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACY1 in samples. An antibody specific for ACY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703122	KTE60906	48 T	$339		Add to Cart
BHE13703122	KTE60906	96 T	$589		Add to Cart
BHE13703122	KTE60906	96 Tx5	$2629		Add to Cart
BHE13703122	KTE60906	96 Tx50	$24569		Add to Cart

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