Human Aminoacylase-1 - ACY1 ELISA Kit

Mfr.No.

E4113hu

Sensitivity

0.106ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Aminoacylase-1 (also known as ACY1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ACY1;ACY-1;ACY1D;Aminoacylase-1;HEL-S-5;N-acyl-L-amino-acid amidohydrolase

Molecular Weight

45885

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4113hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.18	0.71	3.52
Intra-Assay	2	18	33.78	0.89	2.64
Intra-Assay	3	18	13.07	0.62	4.77

Background

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).

Protocol

These standard curves of E4113hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	1.987	1.852	1.798
	1.717
20ng/ml	0.877	0.96	0.906
	1.043
10ng/ml	0.623	0.584	0.53
	0.545
5ng/ml	0.206	0.221	0.166
	0.235
2.5ng/ml	0.107	0.114	0.06
	0.121
0ng/ml	0.05	0.054	0
	0.058

Gene ID	95
UniProt ID	Q03154

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105518	E4113hu	48T	$320		Add to Cart
BHE12105518	E4113hu	96T	$458		Add to Cart

Human Aminoacylase-1 - ACY1 ELISA Kit

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