Matrices listed below were spiked with certain level of AMDHD2 and the recovery rates were calculated by comparing the measured value to the expected amount of AMDHD2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
94
EDTA plasma(n=5)
95-103
99
heparin plasma(n=5)
94-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMDHD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-98%
85-103%
85-103%
EDTA plasma(n=5)
86-100%
83-101%
83-99%
heparin plasma(n=5)
83-99%
87-98%
89-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9Y303
Applications
ELISA
Applications Notes
AMDHD2 ELISA Kit allows for the in vitro quantitative determination of AMDHD2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human AMDHD2 (N-acetylglucosamine-6-phosphate deacetylase) ELISA Kit