Human alpha2-Antiplasmin - A2-AP ELISA Kit

Mfr.No.

E0964Hu

Description

This sandwich kit is for the accurate quantitative detection of human Alpha 2-Antiplasmin (also known as A2-AP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Alpha-2-antiplasmin;Alpha-2-AP;Alpha-2-PI;Alpha-2-plasmin inhibitor;Serpin F2;SERPINF 2;SERPINF2

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

54566

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.07ng/L

Background

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

Gene ID	5345
UniProt ID	P08697
Antigen	SERPINF2

Research Area

Others

Protocol

These standard curves of E0964Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.818	1.905	1.869
	1.993
600ng/L	1.144	1.063	1.026
	0.982
300ng/L	0.564	0.504	0.468
	0.444
150ng/L	0.159	0.22	0.183
	0.28
75ng/L	0.109	0.121	0.085
	0.133
0ng/L	0.025	0.036	0
	0.048

Precision

We measured random samples of E0964Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	864.9	28.25	3.3
Intra-Assay	2	18	506.8	24.41	4.8
Intra-Assay	3	18	327.7	15.11	4.6

Storage	2-8°C

Reference	Dysfunctional fibrinolysis and cerebral venous thrombosis Journal: Blood Cells Mol Dis. 2017 Jun;65:51-55.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102638	E0964Hu	48T	$320		Add to Cart
BHE12102638	E0964Hu	96T	$458		Add to Cart

Human alpha2-Antiplasmin - A2-AP ELISA Kit

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