Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

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Mfr.No.

CSB-EL015414HU

Sensitivity

0.156 ng/mL

Reactivity

Human

Sample Type

Saliva, Serum, Urine, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

N-acetylgalactosaminidase, alpha-, D22S674, GALB, Acetylgalactosaminidase, alpha-N- (alpha-galactosidase B), OTTHUMP00000199655, OTTHUMP00000199657, alpha-N-acetylgalactosaminidase

Recovery

The recovery of human ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-104
EDTA plasma (n=4)	103	98-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-105
1:2	Average %	90
	Range %	84-95
1:4	Average %	99
	Range %	95-102
1:8	Average %	97
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.785	2.799	2.792	2.636
25	2.457	2.498	2.478	2.322
12.5	1.982	1.999	1.991	1.835
6.25	1.272	1.291	1.282	1.126
3.12	0.775	0.798	0.787	0.631
1.56	0.533	0.503	0.518	0.362
0.78	0.331	0.356	0.344	0.188
0	0.155	0.157	0.156	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P17050
Antigen	N-acetylgalactosaminidase, alpha-

Applications	ELISA
Research Area	NAGA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501152	CSB-EL015414HU	96T	$695	Add to Cart
BHE10501152	CSB-EL015414HU	5x96T	$3336	Add to Cart
BHE10501152	CSB-EL015414HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.