Human alpha-galactosidase,alphaGAL ELISA Kit

Human alpha-galactosidase,alphaGAL ELISA Kit

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Mfr.No.

CSB-E09457h

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

galactosidase, alpha, GALA, agalsidase alfa, alpha-D-galactosidase A, alpha-D-galactoside galactohydrolase 1, alpha-gal A, alpha-galactosidase A, melibiase, alpha-galactosidase,alphaGAL, alpha-galactosidase,alpha GAL

Recovery

The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	99	98-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-104
1:8	Average %	89
	Range %	82-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
8000	0.224	0.223	0.224
2000	0.362	0.356	0.359
640	0.564	0.557	0.561
160	0.819	0.804	0.812
40	1.174	1.275	1.225
0	2.305	2.404	2.355

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06280

Applications	ELISA
Research Area	GLA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504908	CSB-E09457h	96T	$790	Add to Cart
BHE10504908	CSB-E09457h	5x96T	$3792	Add to Cart
BHE10504908	CSB-E09457h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.