Human Alpha-fetoprotein - AFP ELISA Kit

Mfr.No.

E1630Hu

Sensitivity

0.29ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Alpha-Fetoprotein (also known as AFP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AFP;Alpha-1-fetoprotein;Alpha-fetoglobulin;Alpha-fetoprotein

Molecular Weight

68678

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1630Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	54.1	2.64	4.9
Intra-Assay	2	18	75.7	2	2.6
Intra-Assay	3	18	28.9	0.98	3.4

Background

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Protocol

These standard curves of E1630Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	1.825	1.874	1.842
	1.924
60ng/ml	0.908	1.017	0.984
	1.126
30ng/ml	0.5	0.541	0.508
	0.582
15ng/ml	0.256	0.243	0.21
	0.23
7.5ng/ml	0.162	0.134	0.102
	0.107
0ng/ml	0.028	0.033	0
	0.038

Gene ID	174
UniProt ID	P02771

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103215	E1630Hu	48T	$320		Add to Cart
BHE12103215	E1630Hu	96T	$458		Add to Cart

Human Alpha-fetoprotein - AFP ELISA Kit

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