Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit

Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit

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Mfr.No.

CSB-E17177h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

enolase 1, (alpha), ENO1L1, MBP-1, MPB1, NNE, PPH, 2-phospho-D-glycerate hydro-lyase, MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, non-neural enolase, phosphopyruvate hydratase, tau-crystallin

Recovery

The recovery of mouse EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-100
1:4	Average %	90
	Range %	88-95
1:8	Average %	92
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.812	2.998	2.905	2.743
900	2.215	2.345	2.280	2.118
450	1.803	1.804	1.804	1.642
225	1.210	1.261	1.236	1.074
112.5	0.672	0.720	0.696	0.534
56	0.406	0.431	0.419	0.257
28	0.270	0.251	0.261	0.099
0	0.157	0.166	0.162

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06733

Applications	ELISA
Research Area	ENO1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501146	CSB-E17177h	96T	$790	Add to Cart
BHE10501146	CSB-E17177h	5x96T	$3792	Add to Cart
BHE10501146	CSB-E17177h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.