Human alpha-amylase(AMY1) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14075h

Alternative Names

alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1B, AMY1C

Reactivity

Human

Detection Range

7.8 IU/mL-500 IU/mL

Sample Type

Saliva, Serum, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	88	83-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	103
	Range %	99-107
1:400	Average %	98
	Range %	93-105
1:800	Average %	92
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

1.95 IU/mL

Limit Of Detection

450 nm

UniProt ID	P04745
Antigen	alpha-amylase

Applications

ELISA

Research Area

AMY1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10501138	CSB-E14075h	96T	$790	Add to Cart
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