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Home / ELISA Kit

Human Alpha-Actinin 1 (ACTN-1) ELISA Kit

SKU : BHE13703114

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Alpha-actinin-1 is a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.

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Mfr.No.	KTE60898
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Alpha-Actinin 1 (ACTN-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN1.
Alternative Names	ACTN1; FLJ40884; FLJ54432; F-actin cross-linking protein; actinin 1 smooth muscle; alpha-actinin 1
Detection Method	Colorimetric
Kit Components	Human Alpha-Actinin 1 microplate Human Alpha-Actinin 1 standard Human Alpha-Actinin 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alpha-Actinin 1 (ACTN-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTN1. No significant cross-reactivity or interference between Human ACTN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha-actinin-1 is a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Notes	This Human Alpha-Actinin 1 (ACTN-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN1 in samples. An antibody specific for ACTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	87

Applications	ELISA
Applications Notes	This Human Alpha-Actinin 1 (ACTN-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN1 in samples. An antibody specific for ACTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703114	KTE60898	48 T	$359		Add to Cart
BHE13703114	KTE60898	96 T	$619		Add to Cart
BHE13703114	KTE60898	96 Tx5	$2809		Add to Cart
BHE13703114	KTE60898	96 Tx50	$26209		Add to Cart

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