Human Alpha-2B Adrenergic Receptor

Mfr.No.

E6659Hu

Sensitivity

0.017ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ADRA 2B;ADRA2B;Alpha-2 adrenergic receptor subtype C2;Alpha-2B adrenergic receptor;Alpha-2B adrenoceptor;Alpha-2B adrenoreceptor;Alpha-2BAR

Molecular Weight

49954

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6659Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.3	0.09	3.9
Intra-Assay	2	18	5.3	0.18	3.5
Intra-Assay	3	18	2.1	0.08	3.8

Background

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine ; norepinephrine ; epinephrine = oxymetazoline ; dopamine ; p-tyramine = phenylephrine ; serotonin ; p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine ; chlorpromazine ; phentolamine ; mianserine ; spiperone ; prazosin ; alprenolol ; propanolol ; pindolol.

Protocol

These standard curves of E6659Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.204	2.268	2.233
	2.332
4ng/ml	1.441	1.44	1.404
	1.438
2ng/ml	0.687	0.636	0.601
	0.585
1ng/ml	0.308	0.334	0.299
	0.36
0.5ng/ml	0.202	0.205	0.17
	0.208
0ng/ml	0.04	0.035	0
	0.03

Gene ID	151
UniProt ID	P18089

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115612	E6659Hu	48T	$320		Add to Cart
BHE12115612	E6659Hu	96T	$458		Add to Cart

Human Alpha-2B Adrenergic Receptor - ADRA2B ELISA Kit

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