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Home / ELISA Kit

Human Alpha-1B-glycoprotein (A1BG) ELISA Kit

SKU : BHE13703056

Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Gahne et al. (1987) observed genetic polymorphism of A1B using one-dimensional horizontal polyacrylamide gel electrophoresis followed by Western blotting with specific antiserum. Three different phenotypes, designated 1-1, 1-2, and 2-2, were observed. Family data supported the hypothesis that the three phenotypes are determined by 2 codominant alleles at an autosomal locus.
In pigs the homologous locus is linked to malignant hyperthermia. Several other linkages in pigs and in horses suggest that human chromosomes 19, 6, and 1 are 'candidate chromosomes' for bearing the human A1B.

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Mfr.No.	KTE60840
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Alpha-1B-glycoprotein (A1BG) ELISA Kit employs a two-site sandwich ELISA to quantitate A1BG.
Alternative Names	A1BG; A1B; ABG; DKFZp686F0970; GAB; HYST2477; alpha 1B-glycoprotein
Detection Method	Colorimetric
Kit Components	Human Alpha-1B-glycoprotein microplate Human Alpha-1B-glycoprotein standard Human Alpha-1B-glycoprotein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alpha-1B-glycoprotein (A1BG) ELISA Kit has high sensitivity and excellent specificity for detection of Human A1BG. No significant cross-reactivity or interference between Human A1BG and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Gahne et al. (1987) observed genetic polymorphism of A1B using one-dimensional horizontal polyacrylamide gel electrophoresis followed by Western blotting with specific antiserum. Three different phenotypes, designated 1-1, 1-2, and 2-2, were observed. Family data supported the hypothesis that the three phenotypes are determined by 2 codominant alleles at an autosomal locus. In pigs the homologous locus is linked to malignant hyperthermia. Several other linkages in pigs and in horses suggest that human chromosomes 19, 6, and 1 are 'candidate chromosomes' for bearing the human A1B.
Notes	This Human Alpha-1B-glycoprotein (A1BG) ELISA Kit employs a two-site sandwich ELISA to quantitate A1BG in samples. An antibody specific for A1BG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA1BG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A1BG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A1BG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	518955

Applications	ELISA
Applications Notes	This Human Alpha-1B-glycoprotein (A1BG) ELISA Kit employs a two-site sandwich ELISA to quantitate A1BG in samples. An antibody specific for A1BG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA1BG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A1BG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A1BG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703056	KTE60840	48 T	$339		Add to Cart
BHE13703056	KTE60840	96 T	$589		Add to Cart
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