Human Aldose Reductase(AR)ELISA Kit

Human Aldose Reductase(AR)ELISA Kit

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Mfr.No.

CSB-E16266h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

aldo-keto reductase family 1, member B1 (aldose reductase), ADR, ALDR1, ALR2, AR, MGC1804, Lii5-2 CTCL tumor antigen, aldehyde reductase 1, aldo-keto reductase family 1, member B1, aldose reductase, low Km aldose reductase

Recovery

The recovery of mouse Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-108
EDTA plasma (n=4)	96	88-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	95
	Range %	87-103
1:400	Average %	92
	Range %	84-105
1:800	Average %	96
	Range %	83-106
1:1600	Average %	93
	Range %	88-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
10	2.672	2.711	2.692	2.559
5	1.958	2.013	1.986	1.853
2.5	1.275	1.282	1.279	1.146
1.25	0.729	0.764	0.747	0.614
0.625	0.437	0.434	0.436	0.303
0.312	0.365	0.349	0.357	0.224
0.156	0.259	0.247	0.253	0.120
0	0.130	0.135	0.133

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15121

Applications	ELISA
Research Area	AKR1B1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10501122	CSB-E16266h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.