Human Aldo-keto Reductase Family 1 - Member B10

Mfr.No.

E3401Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Aldo-keto Reductase Family 1 Member B10 (also known as AKR1B10) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AKR1B1;AKR1B10;Aldo-keto reductase family 1 member B10;Aldose reductase-like;Aldose reductase-related protein;ARL-1;ARP;hARP;SI reductase;Small intestine reductase

Molecular Weight

36020

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.1	0.07	6.2
Intra-Assay	2	18	3.5	0.2	5.8
Intra-Assay	3	18	0.9	0.07	7.6

Background

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed: 9565553, PubMed: 18087047, PubMed: 12732097, PubMed: 19013440, PubMed: 19563777). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed: 12732097, PubMed: 18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed: 19013440, PubMed: 19563777). Displays no reductase activity towards glucose (PubMed: 12732097).

Protocol

These standard curves of E3401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.048	2.105	2.055
	2.163
3.2ng/ml	1.643	1.647	1.597
	1.651
1.6ng/ml	1.023	1.015	0.965
	1.007
0.8ng/ml	0.729	0.754	0.704
	0.778
0.4ng/ml	0.411	0.422	0.372
	0.433
0ng/ml	0.06	0.05	0
	0.04

Gene ID	57016
UniProt ID	O60218

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104929	E3401Hu	48T	$320		Add to Cart
BHE12104929	E3401Hu	96T	$458		Add to Cart

Human Aldo-keto Reductase Family 1 - Member B10 - AKR1B10 ELISA Kit

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