Human ALDH18A1(Aldehyde Dehydrogenase 18 Family Member A1) ELISA Kit

Mfr.No.

ELK8545

Alternative Names

Aldehyde dehydrogenase 18A1; Aldehyde dehydrogenase 18 family member A1

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant ALDH18A1 and the recovery rates were calculated by comparing the measured value to the expected amount of ALDH18A1 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-97%	92%
EDTA plasma(n=5)	87-99%	94%
Heparin plasma(n=5)	84-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALDH18A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-104%	84-98%	95-105%	88-102%
EDTA plasma(n=5)	93-104%	83-96%	95-103%	88-95%
Heparin plasma(n=5)	86-98%	93-106%	89-97%	85-94%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.035	1.967
500.00	1.507	1.439
250.00	1.107	1.039
125.00	0.827	0.759
62.50	0.521	0.453
31.25	0.362	0.294
15.63	0.212	0.144
0.00	0.068	0.000

Sensitivity

6.1 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	P54886
Antigen	ALDH18A1
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALDH18A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALDH18A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALDH18A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALDH18A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	hepatic disease
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200921	ELK8545	48T	$265		Add to Cart
BHE15200921	ELK8545	96T	$376		Add to Cart

Human ALDH18A1(Aldehyde Dehydrogenase 18 Family Member A1) ELISA Kit

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