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Home / ELISA Kit

Human Alcohol dehydrogenase (ADH) ELISA Kit

SKU : BHE13703151

Arginine vasopressin is a hormone found in most mammals, including humans. Vasopressin is a peptide hormone. It is derived from a preprohormone precursor that is synthesized in the hypothalamus and stored in vesicles at the posterior pituitary. Most of it is stored in the posterior pituitary to be released into the blood stream; however, some of it is also released directly into the brain.One of the most important roles of AVP is to regulate the body's retention of water; it is released when the body is dehydrated and causes the kidneys to conserve water, thus concentrating the urine, and reducing urine volume. In high concentrations, it also raises blood pressure by inducing moderate vasoconstriction. In addition, it has a variety of neurological effects on the brain, having been found, for example, to influence pair-bonding in voles.

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Mfr.No.	KTE60935
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A.
Alternative Names	ADH1A; ADH1; ADH; alpha subunit; alcohol dehydrogenase 1 (class I); alpha polypeptide; aldehyde reductase; class I alcohol dehydrogenase; alpha subunit
Detection Method	Colorimetric
Kit Components	Human Alcohol dehydrogenase microplate Human Alcohol dehydrogenase standard Human Alcohol dehydrogenase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alcohol dehydrogenase (ADH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADH1A. No significant cross-reactivity or interference between Human ADH1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Arginine vasopressin is a hormone found in most mammals, including humans. Vasopressin is a peptide hormone. It is derived from a preprohormone precursor that is synthesized in the hypothalamus and stored in vesicles at the posterior pituitary. Most of it is stored in the posterior pituitary to be released into the blood stream; however, some of it is also released directly into the brain.One of the most important roles of AVP is to regulate the body's retention of water; it is released when the body is dehydrated and causes the kidneys to conserve water, thus concentrating the urine, and reducing urine volume. In high concentrations, it also raises blood pressure by inducing moderate vasoconstriction. In addition, it has a variety of neurological effects on the brain, having been found, for example, to influence pair-bonding in voles.
Notes	This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A in samples. An antibody specific for ADH1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	124

Applications	ELISA
Applications Notes	This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A in samples. An antibody specific for ADH1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703151	KTE60935	48 T	$359		Add to Cart
BHE13703151	KTE60935	96 T	$619		Add to Cart
BHE13703151	KTE60935	96 Tx5	$2809		Add to Cart
BHE13703151	KTE60935	96 Tx50	$26209		Add to Cart

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