Human Alanine Aminotransferase,ALT/GPT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10082h

Sensitivity

3.9 uIU/mL

Detection Range

15.6 uIU/mL-1000 uIU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glutamic-pyruvate transaminase (alanine aminotransferase), AAT1, ALT1, GPT1, glutamic pyruvate transaminase, glutamic-alanine transaminase 1

Recovery

The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P24298

Applications	ELISA
Research Area	GPT

Storage	Store at 2-8°C. Please refer to protocol.

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