Human Alanine Aminotransferase,ALT/GPT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10082h

Alternative Names

glutamic-pyruvate transaminase (alanine aminotransferase), AAT1, ALT1, GPT1, glutamic pyruvate transaminase, glutamic-alanine transaminase 1

Reactivity

Human

Detection Range

15.6 uIU/mL-1000 uIU/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

3.9 uIU/mL

Limit Of Detection

450 nm

UniProt ID	P24298
Antigen	glutamic-pyruvate transaminase (alanine aminotransferase)

Applications

ELISA

Research Area

GPT

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10501113	CSB-E10082h	96T	$790	Add to Cart
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BHE10501113	CSB-E10082h	10x96T	$7505	Add to Cart