Matrices listed below were spiked with certain level of AKT2 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-97
94
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-101%
87-105%
88-104%
EDTA plasma(n=5)
85-101%
82-94%
84-96%
heparin plasma(n=5)
81-99%
84-100%
82-93%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P31751
Antigen
AKT2
Applications
ELISA
Applications Notes
AKT2 ELISA Kit allows for the in vitro quantitative determination of AKT2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human AKT2(RAC-beta serine/threonine-protein kinase) ELISA Kit