Human AKT1(RAC-alpha serine/threonine-protein kinase) ELISA Kit

Mfr.No.

EH0532

Alternative Names

PKB ELISA Kit, AKT1 ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, AKT ELISA Kit, PKBMGC99656 ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of AKT1 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-101	97
EDTA plasma(n=5)	87-103	95
heparin plasma(n=5)	88-101	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-104%	93-104%	87-100%
EDTA plasma(n=5)	87-97%	90-101%	82-96%
heparin plasma(n=5)	86-100%	80-91%	84-100%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

PKB/AKT1/PKB alpha/PRKBA/RAC-alpha/AKT/PKBMGC99656/Protein kinase B/Proto-oncogene c-Akt/rac protein kinase alpha/RAC-ALPHA/RAC-alpha serine/threonine-protein kinase/RAC-PK-alpha/RACPKB-ALPHA/v-akt murine thymoma viral oncogene homolog 1

Notes

For Research Use Only

UniProt ID	P31749
Antigen	AKT1

Applications	ELISA
Applications Notes	AKT1 ELISA Kit allows for the in vitro quantitative determination of AKT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804989	EH0532	96T	$400		Add to Cart

Human AKT1(RAC-alpha serine/threonine-protein kinase) ELISA Kit

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