Matrices listed below were spiked with certain level of AKAP12 and the recovery rates were calculated by comparing the measured value to the expected amount of AKAP12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
93
EDTA plasma(n=5)
86-101
93
heparin plasma(n=5)
88-96
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKAP12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AKAP12 ELISA Kit allows for the in vitro quantitative determination of AKAP12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human AKAP12(A Kinase Anchor Protein 12) ELISA Kit