Matrices listed below were spiked with certain level of AIRE and the recovery rates were calculated by comparing the measured value to the expected amount of AIRE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
94
EDTA plasma(n=5)
87-105
99
heparin plasma(n=5)
85-98
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AIRE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AIRE ELISA Kit allows for the in vitro quantitative determination of AIRE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human AIRE(Autoimmune regulator) ELISA Kit