Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine®

Mfr.No.

EK1427

Sensitivity

<4 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.012	0.065	0.113	0.223	0.440	0.814	1.439	2.332

Description

Human AGRP/agouti-related ELISA Kit PicoKine® (96 Tests). Quantitate Human AGRP in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Agouti-related protein;AGRP;AGRT, ART;

Molecular Weight

14440 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-T132

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human AGRP

Subcellular Localization

Secreted . Golgi apparatus lumen .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	15	99	236	13	102	246
Standard deviation	0.63	3.96	10.38	0.68	5.4	12.05
CV (%)	4.2%	4%	4.4%	5.3%	5.3%	4.9%

Protein Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. .

Background

Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	15	18	16	17	16	1.11	6.9%
Sample 2	99	100	93	95	96	2.86	2.9%
Sample 3	236	218	202	233	222	13.53	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	181
Gene Name	AGRP
UniProt ID	O00253
Protein Name	Agouti-related protein
Tissue Specificity	Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.

Applications	ELISA
Research Area	Calcium Signaling,Cancer,Cardiovascular,Endocrine System,Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neurology Process,Neuroscience,Obesity,Pathways and Processes,Redox Metabolism,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001043	EK1427	96 wells/kit	$499		Add to Cart

Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine®

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