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Home / ELISA Kit

Human Agouti Related Protein (AGRP) ELISA Kit

SKU : BHE13703183

Agouti-related protein is a neuropeptide produced in the brain (in the arcuate nucleus of the hypothalamus) by the AgRP/NPY neuron that increases appetite and decreases metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators. Understanding the role AgRP plays in weight gain may assist in developing pharmaceutical models for treating obesity. It was identified independently by two teams based on sequence similarity with Agouti signalling peptide, a protein synthetized in the skin that controls coat color.The appetite stimulating effects of AgRP are inhibited by the hormone leptin. Adipocytes secrete leptin in response to food intake. This hormone acts in the arcuate nucleus and inhibits the AgRP/NPY neuron from releasing orexigenic peptides.

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Mfr.No.	KTE60967
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Agouti Related Protein (AGRP) ELISA Kit employs a two-site sandwich ELISA to quantitate AGRP.
Alternative Names	AGRP; AGRT; ART; ASIP2; MGC118963; agouti related protein
Detection Method	Colorimetric
Kit Components	Human Agouti Related Protein microplate Human Agouti Related Protein standard Human Agouti Related Protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Agouti Related Protein (AGRP) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGRP. No significant cross-reactivity or interference between Human AGRP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Agouti-related protein is a neuropeptide produced in the brain (in the arcuate nucleus of the hypothalamus) by the AgRP/NPY neuron that increases appetite and decreases metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators. Understanding the role AgRP plays in weight gain may assist in developing pharmaceutical models for treating obesity. It was identified independently by two teams based on sequence similarity with Agouti signalling peptide, a protein synthetized in the skin that controls coat color.The appetite stimulating effects of AgRP are inhibited by the hormone leptin. Adipocytes secrete leptin in response to food intake. This hormone acts in the arcuate nucleus and inhibits the AgRP/NPY neuron from releasing orexigenic peptides.
Notes	This Human Agouti Related Protein (AGRP) ELISA Kit employs a two-site sandwich ELISA to quantitate AGRP in samples. An antibody specific for AGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	181

Applications	ELISA
Applications Notes	This Human Agouti Related Protein (AGRP) ELISA Kit employs a two-site sandwich ELISA to quantitate AGRP in samples. An antibody specific for AGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703183	KTE60967	48 T	$399		Add to Cart
BHE13703183	KTE60967	96 T	$739		Add to Cart
BHE13703183	KTE60967	96 Tx5	$3339		Add to Cart
BHE13703183	KTE60967	96 Tx50	$29639		Add to Cart

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