Human Afamin - AFM ELISA Kit

Mfr.No.

E3471Hu

Sensitivity

2.38ng/ml

Detection Range

5-600ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Afamin (also known as AFM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AFAMIN;Alpha-Alb;Alpha-albumin

Molecular Weight

69069

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3471Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	148.5	5.89	4
Intra-Assay	2	18	213.8	6.58	3.1
Intra-Assay	3	18	96.7	6.25	6.5

Background

Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed: 26902720). Binds vitamin E (PubMed: 15952736, PubMed: 12463752). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed: 15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed: 19046407).

Protocol

These standard curves of E3471Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	1.872	1.892	1.862
	1.912
160ng/ml	0.979	0.957	0.927
	0.934
80ng/ml	0.537	0.56	0.53
	0.583
40ng/ml	0.222	0.242	0.213
	0.263
20ng/ml	0.14	0.104	0.074
	0.068
0ng/ml	0.026	0.03	0
	0.033

Gene ID	173
UniProt ID	P43652

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104977	E3471Hu	48T	$320		Add to Cart
BHE12104977	E3471Hu	96T	$458		Add to Cart

Human Afamin - AFM ELISA Kit

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