Human Adipsin ELISA Kit

Human Adipsin ELISA Kit

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Mfr.No.

CSB-E14369h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement factor D (adipsin), ADIPSIN, ADN, DF, PFD, C3 convertase activator, D component of complement (adipsin), complement factor D, complement factor D preproprotein, properdin factor D

Recovery

The recovery of human ?-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	93
	Range %	88-94
1:4	Average %	105
	Range %	100-112
1:8	Average %	86
	Range %	80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.838	2.972	2.905	2.782
50	2.140	2.175	2.158	2.035
25	1.416	1.406	1.411	1.288
12.5	0.903	0.895	0.899	0.776
6.25	0.516	0.502	0.509	0.386
3.12	0.330	0.353	0.342	0.219
1.56	0.264	0.281	0.273	0.150
0	0.119	0.127	0.123

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00746

Applications	ELISA
Research Area	CFD

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501069	CSB-E14369h	96T	$595	Add to Cart
BHE10501069	CSB-E14369h	5x96T	$2856	Add to Cart
BHE10501069	CSB-E14369h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.