Human Adipose Triglyceride Lipase,ATGL ELISA kit

Human Adipose Triglyceride Lipase,ATGL ELISA kit

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Mfr.No.

CSB-E12688h

Sensitivity

1.56 mIU/mL

Reactivity

Human

Sample Type

Serum, Urine, Tissue homogenates

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

patatin-like phospholipase domain containing 2, 1110001C14Rik, ATGL, DESNUTRIN, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase

Recovery

The recovery of human SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	101
	Range %	95-106
1:8	Average %	98
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.017	2.063	2.040	1.795
5	1.499	1.472	1.486	1.241
2.5	1.004	1.064	1.034	0.789
1.25	0.705	0.742	0.724	0.479
0.625	0.501	0.536	0.519	0.274
0.312	0.412	0.438	0.425	0.180
0.156	0.347	0.361	0.354	0.109
0	0.241	0.249	0.245	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q96AD5
Antigen	patatin-like phospholipase domain containing 2

Applications	ELISA
Research Area	PNPLA2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501068	CSB-E12688h	96T	$595	Add to Cart
BHE10501068	CSB-E12688h	5x96T	$2856	Add to Cart
BHE10501068	CSB-E12688h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.