Human adiponectin,ADP ELISA Kit

Mfr.No.

CSB-E07270h

Sensitivity

1.102 ng/mL

Detection Range

1.562 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

adiponectin, C1Q and collagen domain containing, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047, OTTHUMP00000210048, adipocyte, C1Q and collagen domain containing, adipocyte, C1Q and collagen domain-containing, adipo

Recovery

The recovery of human LEFTY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LEFTY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.811	2.836	2.824	2.744
250	2.038	2.000	2.019	1.939
125	1.331	1.368	1.350	1.270
62.5	0.804	0.752	0.778	0.698
31.2	0.473	0.498	0.486	0.406
15.6	0.278	0.281	0.280	0.200
7.8	0.173	0.169	0.171	0.091
0	0.079	0.081	0.080	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q15848

Applications	ELISA
Research Area	ADP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501066	CSB-E07270h	96T	$495	Add to Cart
BHE10501066	CSB-E07270h	5x96T	$2376	Add to Cart
BHE10501066	CSB-E07270h	10x96T	$4702.50	Add to Cart

Human adiponectin,ADP ELISA Kit

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