Human Adipolin - AD ELISA Kit

Mfr.No.

E3692Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Adipolin (also known as AD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Adipolin;Adipolin cleaved form;Adipolin fC1QTNF12;Adipolin fCTRP12;Adipolin full-length form;Adipolin gC1QTNF12;Adipolin gCTRP12;Adipose-derived insulin-sensitizing factor;C 1QTNF12;C1q and TNF related protein 12;C1QTNF12;Complement C1q tumor necrosis factor-related protein 12

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32416

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/ml

Background

Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.

Gene ID	388581
UniProt ID	Q5T7M4
Antigen	C1QTNF12

Research Area

Cell Biology

Protocol

These standard curves of E3692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.222	2.239	2.182
	2.257
6ng/ml	1.34	1.362	1.304
	1.384
3ng/ml	0.894	0.862	0.804
	0.83
1.5ng/ml	0.411	0.437	0.379
	0.462
0.75ng/ml	0.257	0.273	0.215
	0.288
0ng/ml	0.065	0.058	0
	0.05

Precision

We measured random samples of E3692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.7	0.1	6.1
Intra-Assay	2	18	5.1	0.33	6.5
Intra-Assay	3	18	5.6	0.23	4.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105159	E3692Hu	48T	$320		Add to Cart
BHE12105159	E3692Hu	96T	$458		Add to Cart

Human Adipolin - AD ELISA Kit

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