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Home / ELISA Kit

Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit

SKU : BHE13703169

AEBP1 binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells. The deduced 1,158-amino acid ACLP protein contains a putative signal peptide; an 11-residue lys/pro-rich motif repeated 4 times in the N terminus; a domain with 30% identity to the slime mold adhesion protein discoidin I; and a C-terminal 500-amino acid domain with 39% identity to carboxypeptidase E (CPE).

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Mfr.No.	KTE60953
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate AEBP1.
Alternative Names	AEBP1; ACLP; FLJ33612; AE-binding protein 1; adipocyte enhancer binding protein 1; aortic carboxypeptidase-like protein
Detection Method	Colorimetric
Kit Components	Human Adipocyte enhancer-binding protein 1 microplate Human Adipocyte enhancer-binding protein 1 standard Human Adipocyte enhancer-binding protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AEBP1. No significant cross-reactivity or interference between Human AEBP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	AEBP1 binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells. The deduced 1,158-amino acid ACLP protein contains a putative signal peptide; an 11-residue lys/pro-rich motif repeated 4 times in the N terminus; a domain with 30% identity to the slime mold adhesion protein discoidin I; and a C-terminal 500-amino acid domain with 39% identity to carboxypeptidase E (CPE).
Notes	This Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate AEBP1 in samples. An antibody specific for AEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AEBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	165

Applications	ELISA
Applications Notes	This Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate AEBP1 in samples. An antibody specific for AEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AEBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703169	KTE60953	48 T	$339		Add to Cart
BHE13703169	KTE60953	96 T	$589		Add to Cart
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BHE13703169	KTE60953	96 Tx50	$24569		Add to Cart

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