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Home / ELISA Kit

Human Adenylate cyclase type 2 (ADCY2) ELISA Kit

SKU : BHE13703145

Adenylate cyclase type 2 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex.
The amino acid sequence of ADCY2 displayed significant homology with ADCY8 in the highly conserved adenylyl cyclase domain (250 amino acids) found in the 3-prime cytoplasmic portion of all mammalian adenylyl cyclases. However, outside this domain, the homology was extremely low.Semiquantitative RT-PCR detected ADCY2 in all tissues examined except peripheral blood leukocytes. Highest expression was detected in brain, skeletal muscle, and testis.

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Mfr.No.	KTE60929
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Adenylate cyclase type 2 (ADCY2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY2.
Alternative Names	ADCY2; AC2; FLJ16822; FLJ45092; HBAC2; KIAA1060; MGC133314; 3 ;5 -cyclic AMP synthetase; ATP pyrophosphate-lyase; adenylate cyclase 2; adenylate cyclase; adenylyl cyclase 2; type adenylate cyclase
Detection Method	Colorimetric
Kit Components	Human Adenylate cyclase type 2 microplate Human Adenylate cyclase type 2 standard Human Adenylate cyclase type 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Adenylate cyclase type 2 (ADCY2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADCY2. No significant cross-reactivity or interference between Human ADCY2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Adenylate cyclase type 2 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex. The amino acid sequence of ADCY2 displayed significant homology with ADCY8 in the highly conserved adenylyl cyclase domain (250 amino acids) found in the 3-prime cytoplasmic portion of all mammalian adenylyl cyclases. However, outside this domain, the homology was extremely low.Semiquantitative RT-PCR detected ADCY2 in all tissues examined except peripheral blood leukocytes. Highest expression was detected in brain, skeletal muscle, and testis.
Notes	This Human Adenylate cyclase type 2 (ADCY2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY2 in samples. An antibody specific for ADCY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	108

Applications	ELISA
Applications Notes	This Human Adenylate cyclase type 2 (ADCY2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY2 in samples. An antibody specific for ADCY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703145	KTE60929	48 T	$339		Add to Cart
BHE13703145	KTE60929	96 T	$589		Add to Cart
BHE13703145	KTE60929	96 Tx5	$2629		Add to Cart
BHE13703145	KTE60929	96 Tx50	$24569		Add to Cart

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