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Home / ELISA Kit

Human Adenosylhomocysteinase (AHCY) ELISA Kit

SKU : BHE13703188

S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia.
In eukaryotes, this is the major route for disposal of the S-adenosylhomocysteine formed as a common product of each of many S-adenosylmethionine-dependent methyltransferases. The reaction is reversible, but under normal conditions the removal of both adenosine and homocysteine is sufficiently rapid to maintain the flux in the direction of hydrolysis.

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Mfr.No.	KTE60972
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Adenosylhomocysteinase (AHCY) ELISA Kit employs a two-site sandwich ELISA to quantitate AHCY.
Alternative Names	AHCY; SAHH; S-adenosylhomocysteine hydrolase
Detection Method	Colorimetric
Kit Components	Human Adenosylhomocysteinase microplate Human Adenosylhomocysteinase standard Human Adenosylhomocysteinase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Adenosylhomocysteinase (AHCY) ELISA Kit has high sensitivity and excellent specificity for detection of Human AHCY. No significant cross-reactivity or interference between Human AHCY and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. In eukaryotes, this is the major route for disposal of the S-adenosylhomocysteine formed as a common product of each of many S-adenosylmethionine-dependent methyltransferases. The reaction is reversible, but under normal conditions the removal of both adenosine and homocysteine is sufficiently rapid to maintain the flux in the direction of hydrolysis.
Notes	This Human Adenosylhomocysteinase (AHCY) ELISA Kit employs a two-site sandwich ELISA to quantitate AHCY in samples. An antibody specific for AHCY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAHCY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AHCY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHCY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	191

Applications	ELISA
Applications Notes	This Human Adenosylhomocysteinase (AHCY) ELISA Kit employs a two-site sandwich ELISA to quantitate AHCY in samples. An antibody specific for AHCY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAHCY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AHCY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHCY bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703188	KTE60972	48 T	$339		Add to Cart
BHE13703188	KTE60972	96 T	$589		Add to Cart
BHE13703188	KTE60972	96 Tx5	$2629		Add to Cart
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