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Home / ELISA Kit

Human Adenosine receptor A2b (ADORA2B) ELISA Kit

SKU : BHE13703161

Adenosine receptors serve as important mediators of a diversity of physiologic processes throughout the body. These receptors are members of the G protein-coupled receptor superfamily, a class of cell-surface receptors that, when activated, couple to a heterotrimeric G protein complex to effect signal transduction.
ADORA2b encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.

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Mfr.No.	KTE60945
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Adenosine receptor A2b (ADORA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA2B.
Alternative Names	ADORA2B; ADORA2;
Detection Method	Colorimetric
Kit Components	Human Adenosine receptor A2b microplate Human Adenosine receptor A2b standard Human Adenosine receptor A2b detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Adenosine receptor A2b (ADORA2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADORA2B. No significant cross-reactivity or interference between Human ADORA2B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Adenosine receptors serve as important mediators of a diversity of physiologic processes throughout the body. These receptors are members of the G protein-coupled receptor superfamily, a class of cell-surface receptors that, when activated, couple to a heterotrimeric G protein complex to effect signal transduction. ADORA2b encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.
Notes	This Human Adenosine receptor A2b (ADORA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA2B in samples. An antibody specific for ADORA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADORA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	136

Applications	ELISA
Applications Notes	This Human Adenosine receptor A2b (ADORA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA2B in samples. An antibody specific for ADORA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADORA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703161	KTE60945	48 T	$339		Add to Cart
BHE13703161	KTE60945	96 T	$589		Add to Cart
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BHE13703161	KTE60945	96 Tx50	$24569		Add to Cart

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