Human adenosine deaminase,ADA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09613h

Alternative Names

adenosine deaminase, adenosine aminohydrolase, adenosine deaminase, adenosine aminohydrolase

Reactivity

Human

Detection Range

1.56 mIU/mL-100 mIU/mL

Sample Type

Tissue homogenates, Serum, Urine

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human FNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-91
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-91
1:2	Average %	106
	Range %	101-109
1:4	Average %	88
	Range %	85-92
1:8	Average %	96
	Range %	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.39 mIU/mL

Limit Of Detection

450 nm

UniProt ID	P00813
Antigen	Adenosine Deaminase

Applications

ELISA

Research Area

ADA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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