Human ADAMTS13 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0927

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.079	0.208	0.295	0.480	0.733	1.228	1.649	2.001

Description

Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ADAMTS13 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate).

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 13;ADAM-TS 13;ADAM-TS13;ADAMTS-13;3.4.24.87;von Willebrand factor-cleaving protease;vWF-CP;vWF-cleaving protease;ADAMTS13;C9orf8;UNQ6102/PRO20085;

Molecular Weight

153604 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ADAMTS13

Subcellular Localization

Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	5.62	10.1	25.5	6.23	12.1	26.4
Standard deviation	0.259	0.444	1.07	0.467	0.835	1.95
CV (%)	4.6%	4.4%	4.2%	7.5%	6.9%	7.4%

Protein Function

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .

Background

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	11093
Gene Name	ADAMTS13
UniProt ID	Q76LX8
Protein Name	A disintegrin and metalloproteinase with thrombospondin motifs 13
Tissue Specificity	Plasma. Expressed primarily in liver. .

Applications	ELISA
Research Area	Cancer,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000019	EZ0927	5 plates/kit	$500		Add to Cart

Human ADAMTS13 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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