Human ADAM9 ELISA Kit PicoKine®

Mfr.No.

EK1341

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.092	0.121	0.194	0.319	0.570	1.023	2.010

Description

Human ADAM9 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAM9 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Disintegrin and metalloproteinase domain-containing protein 9;ADAM 9;3.4.24.-;Cellular disintegrin-related protein;Meltrin-gamma;Metalloprotease/disintegrin/cysteine-rich protein 9;Myeloma cell metalloproteinase;ADAM9;KIAA0021, MCMP, MDC9, MLTNG;

Molecular Weight

90556 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A29-D697

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ADAM9

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	38	260	407	38	269	375
Standard deviation	2.35	16%	17.9	2.43	19.09	20.62
CV (%)	6.2%	6%	4.4%	6.4%	7.1%	5.5%

Protein Function

Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. .

Background

Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM ('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	38	41	35	42	39	2.73	7%
Sample 2	260	225	245	259	247	14.14	5.7%
Sample 3	407	428	485	443	440	28.56	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8754
Gene Name	ADAM9
UniProt ID	Q13443
Protein Name	Disintegrin and metalloproteinase domain-containing protein 9
Tissue Specificity	Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. .
Sequence	Contains 1 disintegrin domain.

Applications	ELISA
Research Area	Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000935	EK1341	96 wells/kit	$499		Add to Cart

Human ADAM9 ELISA Kit PicoKine®

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