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Human Acylglycerol kinase, mitochondrial (AGK) ELISA Kit

SKU : BHE13703179

MULK contains an N-terminal diacylglycerol (DG) kinase domain, a putative nuclear localization signal, and a region with 44% homology to SPHK2. Comparison of putative vertebrate MULK orthologs showed strong conservation of amino acid sequences, and phylogenetic analysis of lipid kinases demonstrated that human and mouse Mulk together form a distinct lipid kinase family.
Expression of a MULK-EGFP fusion protein in 293T cells and CHO cells showed that MULK fractionates with membranes and localizes to internal membrane compartments. RT-PCR analysis showed MULK is ubiquitously expressed, with highest expression in pancreas and brain.

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Mfr.No.	KTE60963
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Acylglycerol kinase, mitochondrial (AGK) ELISA Kit employs a two-site sandwich ELISA to quantitate AGK.
Alternative Names	AGK; FLJ10842; MULK; multi-substrate lipid kinase; multiple substrate lipid kinase
Detection Method	Colorimetric
Kit Components	Human Acylglycerol kinase, mitochondrial microplate Human Acylglycerol kinase, mitochondrial standard Human Acylglycerol kinase, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Acylglycerol kinase, mitochondrial (AGK) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGK. No significant cross-reactivity or interference between Human AGK and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MULK contains an N-terminal diacylglycerol (DG) kinase domain, a putative nuclear localization signal, and a region with 44% homology to SPHK2. Comparison of putative vertebrate MULK orthologs showed strong conservation of amino acid sequences, and phylogenetic analysis of lipid kinases demonstrated that human and mouse Mulk together form a distinct lipid kinase family. Expression of a MULK-EGFP fusion protein in 293T cells and CHO cells showed that MULK fractionates with membranes and localizes to inteal membrane compartments. RT-PCR analysis showed MULK is ubiquitously expressed, with highest expression in pancreas and brain.
Notes	This Human Acylglycerol kinase, mitochondrial (AGK) ELISA Kit employs a two-site sandwich ELISA to quantitate AGK in samples. An antibody specific for AGK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55750

Applications	ELISA
Applications Notes	This Human Acylglycerol kinase, mitochondrial (AGK) ELISA Kit employs a two-site sandwich ELISA to quantitate AGK in samples. An antibody specific for AGK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703179	KTE60963	48 T	$339		Add to Cart
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