Human Acylamino-acid-releasing Enzyme

Mfr.No.

E3613Hu

Sensitivity

9.88ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Heme Oxygenase (also known as HMOX1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AARE;ACPH;Acylamino-acid-releasing enzyme;Acylaminoacyl-peptidase;Acyl-peptide hydrolase;APEH;APH;D3F15S2;D3S48E;OPH;Oxidized protein hydrolase

Molecular Weight

81225

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3613Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	421.79	22.39	5.31
Intra-Assay	2	18	1754.32	76.33	4.35
Intra-Assay	3	18	1056.52	56.25	5.32

Background

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.

Protocol

These standard curves of E3613Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.186	2.065	2.012
	1.945
1200ng/L	1.457	1.412	1.359
	1.366
600ng/L	0.58	0.665	0.612
	0.749
300ng/L	0.361	0.393	0.34
	0.425
150ng/L	0.209	0.185	0.132
	0.162
0ng/L	0.047	0.053	0
	0.059

Gene ID	327
UniProt ID	P13798

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105090	E3613Hu	48T	$320		Add to Cart
BHE12105090	E3613Hu	96T	$458		Add to Cart

Human Acylamino-acid-releasing Enzyme - APEH ELISA Kit

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