Human Acyl-coenzyme A Synthetase Acsm3 - Mitochondrial

Mfr.No.

E4902Hu

Alternative Names

ACSM 3;ACSM3;Acyl-CoA synthetase medium-chain family member 3;Acyl-coenzyme A synthetase ACSM3, mitochondrial;Butyrate--CoA ligase 3;Butyryl-coenzyme A synthetase 3;Middle-chain acyl-CoA synthetase 3;Propionate--CoA ligase;Protein SA homolog;

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

66153

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.23ng/L

Background

Catalyzes the activation of fatty acids by CoA to produce an acyl-CoA, the first step in fatty acid metabolism (PubMed: 11772874). Capable of activating medium-chain fatty acids with a preference for isobutyrate among fatty acids with 2-6 carbon atoms (By similarity).

Gene ID	6296
UniProt ID	Q53FZ2
Antigen	ACSM3

Research Area

Others

Protocol

These standard curves of E4902Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.096	1.957	1.928
	1.817
600ng/L	0.973	1.049	1.021
	1.125
300ng/L	0.58	0.568	0.539
	0.555
150ng/L	0.168	0.181	0.153
	0.194
75ng/L	0.137	0.135	0.107
	0.133
0ng/L	0.027	0.028	0
	0.029

Precision

We measured random samples of E4902Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	428.4	15.39	3.6
Intra-Assay	2	18	747.4	21.27	2.8
Intra-Assay	3	18	294.8	12.87	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106175	E4902Hu	48T	$320		Add to Cart
BHE12106175	E4902Hu	96T	$458		Add to Cart

Human Acyl-coenzyme A Synthetase Acsm3 - Mitochondrial - ACSM3 ELISA Kit

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