Matrices listed below were spiked with certain level of Activin A and the recovery rates were calculated by comparing the measured value to the expected amount of Activin A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
92
EDTA plasma(n=5)
92-105
99
heparin plasma(n=5)
86-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Activin A ELISA Kit allows for the in vitro quantitative determination of Activin A concentrations in serum, plasma, tissue homogenates and other biological fluids.