Human Activating Molecule In Becn1-regulated Autophagy Protein 1

Mfr.No.

E6801Hu

Alternative Names

Activating molecule in BECN1-regulated autophagy protein 1;AMBRA 1;AMBRA1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

142507

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.98ng/L

Background

Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).

Gene ID	55626
UniProt ID	Q9C0C7
Antigen	AMBRA1

Research Area

Neuroscience

Protocol

These standard curves of E6801Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.55	2.545	2.502
	2.54
800ng/L	1.483	1.537	1.494
	1.59
400ng/L	0.924	0.932	0.889
	0.94
200ng/L	0.502	0.497	0.454
	0.493
100ng/L	0.209	0.242	0.199
	0.275
0ng/L	0.043	0.043	0
	0.043

Precision

We measured random samples of E6801Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	289.3	19.03	6.6
Intra-Assay	2	18	617.9	23.92	3.9
Intra-Assay	3	18	519.3	24.24	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115585	E6801Hu	48T	$320		Add to Cart
BHE12115585	E6801Hu	96T	$458		Add to Cart

Human Activating Molecule In Becn1-regulated Autophagy Protein 1 - AMBRA1 ELISA Kit

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