Human activated protein C,APC ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09909h

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

activated protein C,APC

Recovery

The recovery of human FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	101	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	86
	Range %	80-94
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	108
	Range %	98-115
1:8000	Average %	95
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	APC

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Coagulation Factor Hyperfunction After Subarachnoid Hemorrhage Induces Deep Venous Thrombosis Wang Miao.et al,World Neurosurgery,/ Alterations of anticoagulant proteins and soluble endothelial protein C receptor in thalassemia patients of Chinese origin YulanHuang.et al,Thrombosis Research,Octobert 18

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BHE10501039	CSB-E09909h	10x96T	$7505	Add to Cart