Human Activated Leukocyte Cell Adhesion Molecule,ALCAM ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04487h

Sensitivity

0.0097 ng/mL

Detection Range

0.039 ng/mL-2.5 ng/mL

Reactivity

Human

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

activated leukocyte cell adhesion molecule, CD166, FLJ38514, MEMD, MGC71733, activated leucocyte cell adhesion molecule

Recovery

The recovery of human LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	94
	Range %	90-98
1:400	Average %	99
	Range %	95-104
1:800	Average %	95
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13740
Tissue Specificity	Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813). Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771). Detected on peripheral blood monocytes (PubMed:15048703). Detected on monocyte-derived

Applications	ELISA
Research Area	ALCAM

Storage	Store at 2-8°C. Please refer to protocol.

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