Human activated coagulation factor XII,FXIIa ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09232h

Alternative Names

activated coagulation factor XII,FXIIa

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	100-115
1:2	Average %	92
	Range %	84-97
1:4	Average %	100
	Range %	90-104
1:8	Average %	100
	Range %	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

Antigen	activated coagulation factor Ⅻ,FⅫa

Applications

ELISA

Research Area

FXIIa

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10501036	CSB-E09232h	96T	$790	Add to Cart
BHE10501036	CSB-E09232h	5x96T	$3792	Add to Cart
BHE10501036	CSB-E09232h	10x96T	$7505	Add to Cart