Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ACSL4/FACL4 and the recovery rates were calculated by comparing the measured value to the expected amount of ACSL4/FACL4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-98
92
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
85-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACSL4/FACL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-101%
88-102%
85-98%
EDTA plasma(n=5)
82-97%
86-101%
87-98%
heparin plasma(n=5)
80-99%
80-100%
80-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O60488
Applications
ELISA
Applications Notes
ACSL4 ELISA Kit allows for the in vitro quantitative determination of ACSL4/FACL4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ACSL4 (Long-chain-fatty-acid-CoA ligase 4) ELISA Kit