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| Mfr.No. | EH6088 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 18.75pg/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 31.25-2000pg/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Duration | 4 hours | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Type | Sandwich | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ACSL4/FACL4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ACSL4/FACL4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ACSL4/FACL4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | Long-chain-fatty-acid--CoA ligase 4 ELISA Kit, Arachidonate--CoA ligase ELISA Kit, Long-chain acyl-CoA synthetase 4 ELISA Kit, LACS 4 ELISA Kit, ACSL4 ELISA Kit, ACS4 ELISA Kit, FACL4 ELISA Kit, LACS4 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Method | Double Antibody | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kit Components |
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| Recovery | Add a certain amount of ACSL4/FACL4 into the sample. Calculate the recovery by comparing the measured value with the expected amount of ACSL4/FACL4 in the sample.
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| Linearity | Dilute the sample with a certain amount of ACSL4/FACL4 at 1:2, 1:4 and 1:8 to get the recovery range.
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| Specificity | Specifically binds with ACSL4/FACL4 , no obvious cross reaction with other analogues. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Standard Curve | This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
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| Precision | Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
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| Stability | Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
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| Notes | For Research Use Only | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Usage Notes | 
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| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE10808659 | EH6088 | 48T | $280 |
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| BHE10808659 | EH6088 | 96T | $400 |
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