Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

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Mfr.No.

CSB-EL021850HU

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sphingomyelin phosphodiesterase, acid-like 3B, RP11-460I13.1, ASML3B, acid sphingomyelinase-like phosphodiesterase, acid sphingomyelinase-like phosphodiesterase 3B

Recovery

The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	94
	Range %	90-103
1:10000	Average %	96
	Range %	92-100
1:20000	Average %	90
	Range %	85-97
1:40000	Average %	92
	Range %	85-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
7500	0.289	0.312	0.301
1875	0.558	0.542	0.550
468	0.912	0.921	0.917
117	1.612	1.543	1.578
29	1.853	1.934	1.894
0	2.406	2.398	2.402

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q92485

Applications	ELISA
Research Area	SMPDL3B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501029	CSB-EL021850HU	96T	$695	Add to Cart
BHE10501029	CSB-EL021850HU	5x96T	$3336	Add to Cart
BHE10501029	CSB-EL021850HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.