Human acid sphingomyelinase, ASM ELISA Kit

Mfr.No.

CSB-E09361h

Alternative Names

sphingomyelin phosphodiesterase 1, acid lysosomal, ASM, NPD, acid sphingomyelinase

Reactivity

Human

Detection Range

1.25 ng/mL-80 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Urine, Cerebrospinal fluid

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CDKN2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	93	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CDKN2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-112
1:2	Average %	96
	Range %	92-99
1:4	Average %	92
	Range %	86-96
1:8	Average %	94
	Range %	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.31 ng/mL

Limit Of Detection

450 nm

UniProt ID	P17405
Antigen	sphingomyelin phosphodiesterase 1, acid lysosomal

Applications

ELISA

Research Area

SMPD1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501028	CSB-E09361h	96T	$790	Add to Cart
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BHE10501028	CSB-E09361h	10x96T	$7505	Add to Cart