Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

Mfr.No.

ELK1000

Sensitivity

0.16 ng/mL

Detection Range

0.4-25 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

Recovery

Matrices listed below were spiked with certain level of recombinant ACE and the recovery rates were calculated by comparing the measured value to the expected amount of ACE in samples.

Matrix	Recovery range	Average
serum(n=5)	80-90%	85%
EDTA plasma(n=5)	84-106%	100%
Heparin plasma(n=5)	87-93%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	83-92%	92-96%	86-98%	95-101%
EDTA plasma(n=5)	88-96%	86-95%	90-103%	87-90%
Heparin plasma(n=5)	87-94%	85-96%	92-102%	81-90%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
25.00	2.045	1.966
12.50	1.618	1.539
6.25	1.226	1.147
3.13	0.834	0.755
1.57	0.583	0.504
0.79	0.426	0.347
0.40	0.324	0.245
0.00	0.079	0.000

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

25 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACE in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P12821

Applications	ELISA
Research Area	Enzyme & Kinase;Cardiovascular biology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204272	ELK1000	48T	$265		Add to Cart
BHE15204272	ELK1000	96T	$376		Add to Cart

Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

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