Matrices listed below were spiked with certain level of ACC and the recovery rates were calculated by comparing the measured value to the expected amount of ACC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
93
EDTA plasma(n=5)
86-102
93
heparin plasma(n=5)
86-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ACC ELISA Kit allows for the in vitro quantitative determination of ACC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ACC(Acetyl-CoA Carboxylase) ELISA Kit