Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

Mfr.No.

ELK2100

Alternative Names

Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Abeta1-42,amyloid betapeptide 1-42

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-102%	95%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-92%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-96%	79-95%	95-102%
EDTA plasma(n=5)	85-92%	79-96%	87-103%	90-99%
Heparin plasma(n=5)	88-97%	96-105%	91-103%	89-97%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.161	2.078
500.00	1.641	1.558
250.00	1.234	1.151
125.00	0.969	0.886
62.50	0.543	0.460
31.25	0.351	0.268
15.63	0.291	0.208
0.00	0.083	0.000

Sensitivity

5.31 pg/mL

Limit Of Detection

1000 pg/mL

Antigen	Ab1-42
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Neuro science;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203666	ELK2100	48T	$320		Add to Cart
BHE15203666	ELK2100	96T	$458		Add to Cart

Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

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